Optimize the Mutagenesis Strategy by Comparison to Develop High-Yield Alkaline Protease Strains
DOI:
https://doi.org/10.61173/7htwwr53Keywords:
UV, ARTP, alkaline proteaseAbstract
Alkaline protease is a hydrolytic enzyme capable to degrade proteins into small peptides and amino acids, as a biocatalyst, it is applied in various fields such as detergent and leather industry. Here we report the different sources and pH values of alkaline protease and the bacterial species producing alkaline protease, discuss UV and ARTP and compare these two mutagenesis methods, and show that ARTP causes more DNA damnification than other mutagenesis methods and has the highest mutation rate caused by ARTP. In order to improve the protease production of Bacillus strains, the previous study has proposed some new and optimized mutagenesis methods. In addition, this paper demonstrates and highlights the considerable industrial potential of alkaline protease in detergents, leather processing, silver recycling, medical use, food processing, feed, chemical industry, and waste treatment, in which detergent and leather industry are more used.References
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