T cell derived exosome for cancer immunotherapy

Abstract

To date, many approaches to cancer treatment have been developed, such as adoptive cellular immunotherapy and cancer vaccines. However, these methods often have certain limitations, such as low response rates and the risk of autoimmune attacks. Using exosomes for cancer intervention is currently a very promising therapeutic approach, especially for drug delivery or direct cancer cell targeting through exosomes. In this study, we focused on the extraction of exosomes derived from T cells to induce apoptosis in tumors, as well as conducting fundamental tests on their characteristics. We successfully extracted a certain number of exosomes from mouse T cell line CTLL-2 cells and examined their properties, including particle size, zeta potential, electron transmission microscopy, and western blot analysis. Finally, we confirmed the viability of the extracted exosomes in vitro.