Evaluating the Effects of Cinobufagin on Osteosarcoma Using the Saos-2 Cell Line

Abstract

This study explores the potential of cinobufagin in inhibiting osteosarcoma cells using the Saos-2 cell line as a model. The investigation aims to elucidate cinobufagin’s effects on cell viability, phospho-STAT3 levels, tumor size in xenograft models, and cell migration under varying concentrations and treatment durations. The hypothesis proposes that increasing concentrations and treatment duration with cinobufagin can reduce cell viability, tumor size, migration, and phospho-STAT3 activation in osteosarcoma cells. The study used experimental assays such as MTT viability, Western blotting for phospho-STAT3, xenograft tumor models for tumor size, and Boyden chamber assay for cell migration. The results demonstrate that cinobufagin consistently decreases cell viability, inhibits phospho-STAT3 signaling, reduces tumor growth in xenograft models, and impairs cell migration in osteosarcoma cells. These findings support the hypothesis that cinobufagin has multi-faceted anti-cancer effects against osteosarcoma, highlighting its potential as a therapeutic agent in combating this challenging malignancy.