To explore the viability of hematopoietic stem cells in high doses of ionizing radiation

Abstract

Excessive exposure to radiation poses a significant threat to the integrity of our hematopoietic stem cells, which are essential for blood formation. The primary objective of our research is to safeguard the viability of these cells against the onslaught of high-dose radiation. Despite the critical nature of this issue, current literature lacks an in-depth investigation into the mechanisms by which hematopoietic stem cells respond to such intense radiation. To address this gap, we have embarked on a study to modulate the expression levels of the STAT3 protein within hematopoietic stem cells. By employing both gene knockout and overexpression techniques, we aim to elucidate the role of STAT3 in the survival of these cells post-radiation exposure. Our methodology will involve a meticulous enumeration of surviving cells to assess whether the modulation of STAT3 confers a protective advantage against radiation-induced damage.