Circular RNA: Identifying the Synergistic Effects of Circular RNA and miRNA in the Regeneration of Muscle Tissue in an Axolotl

Abstract

Circular RNAs are unique endogenous genes. They maintain various functions, especially in regeneration, including miRNA sponges, interaction with RBPs, etc. Among them, the miRNA sponge is spotlighted. However, our current understanding of the functions of circular RNAs, especially miRNA sponges, mainly focuses on the development and treatment of diseases such as cardiovascular disease, cancers, Alzheimer’s disease, and so on. The knowledge about the role it plays in the regeneration of animals is limited nowadays. To overcome intellectual barriers, I will select axolotl as an ideal model to study the functions of circular RNAs in animal regeneration due to their high regenerative capacity. Although circular RNAs have been proven to play a significant role in the regeneration of axolotl’s limbs, the accurate mechanism in the regeneration of muscle tissue deserves to be studied. In this paper, I aim to discover the connections between miRNA sponge and the regeneration of muscle tissue in axolotl by locating the miRNA in axolotl and comparing the abundance of specific miRNA and relevant circular RNAs in the normal stage and regeneration stage. Ultimately, the purpose of this paper is to assess if the circular RNAs will influence the regeneration of muscle tissue in axolotl and its pathway. The result of this paper may provide a shred of evidence about the regulation of circular RNAs in the regeneration of animals