Research Progress and Future Prospects of Detection Technology Based on ctDNA methylation in Cancer detection
DOI:
https://doi.org/10.61173/jk6ssc07Keywords:
Circulating tumor DNA, Cell-free DNA, Tu-mor, DNA methylationAbstract
CtDNA methylation detection technology, as a novel tumor marker detection method, plays a significant role in cancer screening. This article summarizes the development history of ctDNA methylation detection technology, existing ctDNA methylation detection technology, and practical application cases of ctDNA methylation detection technology in cancer screening. It also analyzes the technical principles of two major existing techniques: affinity enrichment and Em-seq, as well as their respective advantages and disadvantages.
References
[1] Revisionist, F., Tarantino, P. E., Morsel-Abate, A. M., Lamaze, G., Accomplice, E., Carapace, P., Domestically, M., D Notaries, S., Coda, E., & Beardie, M. (2001). Serum alphalipoprotein for diagnosis of extracellular carcinoma in patients with chronic liver disease: influence of Washbag and anti- CV status. Journal of hematology, 34(4), 570–575.HTTP:// do.org/10.1016/s0168-8278(00)00053-2
[2] Leon, S. A., Shapiro, B., Karloff, D. M., & Rosary, M. J. (1977). Free DNA in the serum of cancer patients and the effect of therapy. Cancer research, 37(3), 646–650.
[3] Bhang, L., Anglia, Y., Li, S., Deng, F., Deng, Y., Chen, Z., Li, S., Tao, Y., & Bu, F. (2019). The interplay of circulating tumor DNA and chromatin modification, therapeutic resistance, and metastasis. Molecular cancer, 18(1), 36. HTTP://do.org/10.1186/ s12943-019-0989-z.
[4] Din, Z., & Li, Y. (2018). DNA methylation in human diseases. Genes & diseases, 5(1), 1–8.HTTP://do.org/10.1016/ j.gen dis.2018.01.002
[5] Kankakee Cameraman, C., Tang, K. D., Sassanian, S., Angleton-Lock ton, J., Kenny, L., & Balladeer, C. (2020). DNA methylation Changes in Human Cytomegalovirus-Driven Head and Neck Cancers. Cells, 9(6), 1359. HTTP://do.org/10.3390/ cells9061359.
[6] Sherwood, K., & Weirder, E. T. (2018). Characteristics, properties, and potential applications of circulating cell-free Ana in clinical diagnostics: a focus on transplantation. Journal of immunological methods, 463, 27–38. HTTP://do.org/10.1016/ j.Jim.2018.09.011
[7] HOTCHKISS R. D. (1948). The quantitative separation of purines, pyrimidines, and nucleoside by paper chromatography. The Journal of biological chemistry, 175(1), 315–332.
[8] Thievery, A. R., Cl Message, S., Gan, P. B., Anger, P., & Strong, M. (2016). Origins, structures, and functions of circulating DNA in oncology. Cancer metastasis reviews, 35(3), 347–376. HTTP://do.org/10.1007/s10555-016-9629-x
[9] Chen, X., Dole, J., Gore, A., He, Q., Lu, M., Min, J., Yuan, Z., Yang, X., Jiangsu, Y., Bhang, T., Duo, C., Li, X., Ch eng, L., Bhang, Z., Ni, H., Li, Z., Die, Z., Chi, H., Bhang, X., Fan, M., … Din, L. (2020). Non-invasive early detection of cancer four years before conventional diagnosis using a blood test. Nature communications, 11(1), 3475.HTTP://do.org/10.1038/s41467- 020-17316-z
[10] Fromm, M., McDonald, L. E., Millard, D. S., Colitis, C. M., Watt, F., Gregg, G. W., Moll, P. L., & Paul, C. L. (1992). A ergonomic sequencing protocol that yields a positive display of 5-methylcytosine residues in individual DNA strands. Proceedings of the National Academy of Sciences of the United States of America, 89(5), 1827–1831.
[11] Airship, M., & Shiatsu, H. (2004). High-speed conversion of cytosine to uracil in sulfide ergonomic sequencing analysis of DNA methylation. DNA research : an international journal for rapid publication of reports on genes and genomes, 11(6), 409–415.
[12] Booth, M. J., Bran co, M. R., FICA, G., Huxley, D., Kruger, F., Reek, W., & Balasubramanian, S. (2012). Quantitative sequencing of 5-methylcytosine and 5-hydroxymethylcytosine at single-base resolution. Science (New York, N.Y.), 336(6083), 934–937.HTTP://do.org/10.1126/science.1220671.
[13] Fao, F., Bhang, J., Jiangsu, P., Gong, D., Wang, J. W., Mia, Y., MasterCard, M. V., Wang, J., & Gilding, P. T. (2014). Marked methylation changes in intestinal genes during the perinatal period of preterm neonates. BMC genomics, 15(1), 716. HTTP:// do.org/10.1186/1471-2164-15-716.
[14] Christendom, A. E., Margarita, F., Lecher, M., Bartlett, T., Integer, J., Gomez-Caballero, D., & Beck, S. (2013). A betamixture antiquate normalization method for correcting probe design bias in Illuminance Infinitude 450 k DNA methylation data. Bio informatics (Oxford, England), 29(2), 189–196. HTTP://do.org/10.1093/Bio informatics/bts680.
[15] Li, D., Bhang, B., Ding, X., & Wang, T. (2015). Combining Me Dip-seq and ERE-seq to investigate genome-wide C Pg methylation. Methods (San Diego, Calif.), 72, 29–40.
[16] Keyholes, M. K., & Galbraith, D. W. (2001). High-density micro arrays for gene expression analysis. Optometry, 43(4), 229–238.
[17] Huang, R. S. P., Ciao, J., Patrick, D. C., Duo, C., Yang, L., Din, D. X., Fender, B., Converse, E., Gillian, J. K., Menzies, M., Duncan, D., Lin, D. I., Dennis, L., Airer, A., Grouper, O., Oxnard, G., Vestrymen, J., Elvin, J., Kissogram, S. H., & Ross, J. S. (2021). Circulating Cell-Free DNA Yield and Circulating- Tumor DNA Quantity from Liquid Biopsies of 12 139 Cancer Patients. Clinical chemistry, 67(11), 1554–1566.
[18] Koowattanasuchat, S., Fingernail, S., Ultimatum, P., Thoroughgoing, J., Plainchant, W., Compositor, A., Pakistani, U., Wanna, Y., Interfaith, T., Chootawiriyasakul, K., Ana ta, P., Charmaine, P., Than, R., Insinuation, C., & Pygmalion, T. (2023). Rapid detection of cancer DNA in human blood using steaminess-capped Aunts and a machine learning-enabled smartphone. RSC advances, 13(2), 1301–1311. HTTP:// do.org/10.1039/d2ra05725e.
[19] Li, J. Y., Duo, L. P., Bu, J., & Sun, C. Y. (2024). Clinical applications of circulating tumor DNA in hematological malignancies: From past to the future. Blood reviews, 68, 101237.HTTP://do.org/10.1016/j.bare.2024.101237.
[20] Cheerer, F., Kurt, D. M., Newman, A. M., Ste hr, H., Craig, A. F., Isfahan, M. S., Love joy, A. F., Cabochon, J. J., Class, D. M., Li, C. L., Chou, L., Glover, C., Kisser, B. C., Outsides, G. A., Advance, R. H., Damaged, L. S., Gupta, N. K., Levy, R., Origami, R. S., Chunder, C. A., … Idealize, A. A. (2016). Distinct biological sub types and patterns of genome evolution in lymphoma revealed by circulating tumor DNA. Science transitional medicine, 8(364), 364ra155. HTTP://do.org/10.1126/ Dean&Francis ISSN 2959-409X transliterated.aai8545.
[21] Anshan WANG, Panchayat DUAN. Research progress of liquid biopsy in multiple myeloma[J]. Journal of Shandong First Medical University&Shandong Academy of Medical Sciences. 2024, 45(4): 236-240 HTTP://do.org/10.3969/ j.ISS.2097-0005.2024.04.009
[22] Li, J. Y., Duo, L. P., Bu, J., & Sun, C. Y. (2024). Clinical applications of circulating tumor DNA in hematological malignancies: From past to the future. Blood reviews, 68, 101237.HTTP://do.org/10.1016/j.bare.2024.101237.
[23] Chen Hangmen, Bhang Binning, Chou Yang, and Bu Junkie. Advances in DNA methylation detection for early diagnosis of solid tumors [J]. Industrial Microbiology, 2023,53(4):41-43.
[24] Yang Bin (YEASIN AHAMED). The relationship between SPG20 and SFRP2 gene methylation and collector cancer [D]. Guangzhou University, 2022.DOI:10.27441/d.kink. gypped.2022.001058.
[25] Headlock, P., Chan, A. T., Giovanni, E., Fuchs, C. S., Wu, K., Maharishi, R., O’Brien, M., & Gino, S. (2014). Progress and opportunities in molecular pathological epidemiology of collector malignant lesions. The American journal of Gastroenterology, 109(8), 1205–1214. HTTP://do.org/10.1038/a jg.2014.153.
[26] Frank, S. A. (2007). Dynamics of Cancer: Incidence, Inheritance, and Evolution. Princeton University Press.
[27] Nagy, J. A., Chang, S. H., Dvorak, A. M., & Dvorak, H. F. (2009). Why are tumour blood vessels abnormal and why is it important to know?. British journal of cancer, 100(6), 865–869. HTTP://do.org/10.1038/SJ.JCS.6604929.
[28] Chen, W., Sheng, R., Baaed, P. D., Bhang, S., Deng, H., Bray, F., Female, A., Bu, X. Q., & He, J. (2016). Cancer statistics in China, 2015. CA: a cancer journal for clinicians, 66(2), 115– 132. HTTP://do.org/10.3322/ca ac.21338
[29] Ciao Inyanga. The correlation between circulating tumor DNA methylation and the prognosis of patients with stage II collector cancer after surgery [D]. Henan University, 2024. DOI:10.27114/d.kink.naught.2024.003362.
[30] Tan Ciao. Construction of two types of functional managerial-based biosensors and their preliminary application in the detection of methylation in collector cancer [D]. PLA Army Medical University, 2024.DOI:10.27001/d.kink. gtjyu.2024.000073
[31] ha Jinny. Mining of collector cancer diagnostic markers based on DNA methylation profiles [D]. Harbin Institute of Technology, 2023.DOI:10.27061/d.kink.ghoul.2023.003137.
[32] Bray, F., Farley, J., Aromatherapist, I., Elsie, R. L., Lorre, L. A., & Female, A. (2018). Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA: a cancer journal for clinicians, 68(6), 394–424.HTTP://do.org/10.3322/ca ac.21492.
[33] Man sour, L. A., Cl Crazily, M., Mohamed, A. A., Mahout, E. H., Hammy, S., & Cl Bayed, E. H. (2017). Circulating Hyperventilated RASSF1A as a Molecular Biomarker for Diagnosis of extracellular Carcinoma. Asian Pacific journal of cancer prevention : APJCP, 18(6), 1637–1643. HTTP:// do.org/10.22034/APJCP.2017.18.6.1637.
[34] Wang, J., Yang, L., Ciao, Y., Li, J., Li, J., Li, R., Sheng, L., Bhang, K., Ma, Y., & ha, X. (2021). Circulating tumour DNA methylation in extracellular carcinoma diagnosis using digital droplet PCR. The Journal of international medical research, 49(3), 300060521992962.
[35] Chaos, S., Hong, Y., Bu, X., Wang, Y., Ye, P., CAI, J., Li, Y., Sun, J., Lei, Z., Jiangsu, Y., & Li, J. (2019). H3K4 methylation Regulates ALPS-Induced Inflammatory Cytokines Expression and Release in Macrophages. Shock (Augusta, Ga.), 51(3), 401– 406.
[36] Davy, S., Singalong, J., Singalong, S. S., & Hiawatha, S. (2009). hSET1: a novel approach for colon cancer therapy. Biochemical pharmacology, 77(10), 1635–1641.
[37] Chavez, J. D., & Bruce, J. E. (2019). Chemical crosslinking with mass retrospect: a tool for systems structural biology. Current opinion in chemical biology, 48, 8–18.
[38] Tan, S., Li, R., Ian, X., Duo, X., & Bhang, K. (2021). Se Pu = Chinese journal of chromatography, 39(10), 1094–1101.
[39] Evansville, R., Positional, V. K. C., Sun, Z., Langouste, B. W., Hales, L., Gan, S., Aid, N., Campbell, M. A., Sexton, B. S., Marks, K., Samarkand, M., Samuelson, J. C., Church, H. E., Mahayana, E., Corral, I. R., Jr, Prada, S., Antimalarial, E. T., Evans, T. C., Jr, Williams, L., & Davis, T. B. (2021). Enzymatic methyl sequencing detects DNA methylation at single-base resolution from pictogram of DNA. Genome research, 31(7), 1280–1289.
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