The Application of CRISPR Technology in Tumor-Targeted

Abstract

This article gave an overview for CRISPR-Cas9 technology use and advancement in precise cancer treatment. As the burden of cancer worldwide continues to increase, traditional therapies is plagued by devastating side effects because of their non-cell specificity, and molecularly targeted therapies have the disadvantage of drug resistance and limited application, which cannot adapt to the needs of clinical treatment. In a recent development, the innovative gene-editing tool known as CRISPR has demonstrated great potential in the field of cancer treatment. This study provides an in-depth examination of the mechanisms and potential uses of CRISPR in precision cancer therapy, including the editing of key genes, the rejuvenation of tumor suppressor genes, the modification of immune cells, and the discovery of new treatment targets. Clinical trials showed that CRISPR-modified T cells show tolerable safety profile and limited efficacy to selected patients with hematologic malignancies. The findings showed CRISPR holds the promise to circumvent the limitations of current therapies and deliver novel cancer therapy. Yet, the off-target effect, the inefficient delivery, and complexity of the tumor microenvironment are significant barriers for clinical application. Future work includes how to increase editing precision and safety, adapt it to other tumor types, as well as combination strategy with other therapeutic approaches to elevate its clinical application towards precision oncology.