Nanopore Technology for Future Point-of-Care Testing (POCT): System Integration and Commercialization Prospects
DOI:
https://doi.org/10.61173/ntseed74Keywords:
Point-of-care diagnostic testing (POCT), Multiplex detection capability, Nanopore-based sensing, Engineering monetization processAbstract
The potential applications and potential business applications for nanopore sensing systems in point-of-care tests (POCT) will be covered in detail in this paper. With the COVID-19 crisis exposing the limitations of traditional point-of-care testing methods like LFA in terms of awareness, nanopore technology, with its benefits of no tagging, real-time recognition, and single-molecule resolution, has developed into a potent candidate to bridge the gap between POCT and high-sensitivity lab testing. The paper introduces the fundamental principle of nanopore sensing and its two detection strategies, direct detection and carrier-controlled detection, which both apply to various complex sample environments. The article provides further details on the POCT system integration process, including the inclusion of high-speed and low-noise electrical equipment, a smooth link with smartphones and sky platforms, and the design of microfluidic chips for test pretreatment, flexible solid-state nanopore arrays, and system integration. The paper assesses the challenges faced during commercialization, such as sample interference, pore blockage, and technical bottlenecks caused by difficult economic noise. It looks forward to solving these problems through fresh materials, edge AI, and low-power devices. Ultimately, nanopore technology has much potential for quick, sensitive, and multi-target point-of-care testing in neighborhoods, pharmacies, homes, and even far-off places. It provides a crucial technological foundation for marketing distributed open health control.
References
fective designs will make diseases like Ebola more acces- [1] Shi, W., Friedman, A. K., & Baker, L. A. (2017). Nanopore sible, making it easier for non-experts to observe them. sensing. Analytical Chemistry, 89(1), 157–188. https://doi. org/10.1021/acs.analchem.6b04260 [2] Pugh J. (2023). The Current State of Nanopore 6. Conclusion Sequencing. Methods in molecular biology (Clifton, N.J.), 2632, This paper conducts an in-depth discussion on the app 3–14. https://doi.org/10.1007/978-1-0716-2996-3_1 prospects of nanopore technology in the coming POCT. [3] Wang, Y., Zhao, Y., Bollas, A., Wang, Y., & Au, K. F.
Traditional rapid diagnostic techniques, like LFA, have (2021). Nanopore sequencing technology, bioinformatics and considerable sensitivity issues, despite their simplicity of applications. Nature Biotechnology, 39, 1348–1365. https://doi. use. They are more vulnerable to producing false negative org/10.1038/s41587-021-01108-x effects, especially in the early stages of infections with [4] Chinappi, M., Di Muccio, G., Giordani, C., Cecconi, F., &
small viral loads, thus affecting the effectiveness of the Rocca, B. M. (2022). A Brownian computational approach for incident power. In comparison, nanopore technology can supporting the design of nanopore-based biosensors. In 2022 be used in rural areas or areas with limited resources, with IEEE International Workshop on Metrology for Industry 4.0 & its distinct advantages including the absence of labeling IoT (pp. 98–103). IEEE. https://doi.org/10.1109/METROIND4.0 Dean&Francis ISSN 2959-409X
IOT54413.2022.9831727 [10] Kangarshahi, B. M., & Naghib, S. M. (2024). [5] He, S., Wei, X., Dong, W., Pu, Y., Yang, L., Liu, L., Huang, T., Nanogenosensors based on aptamers and peptides for
Fu, B., Zhang, Z., Liu, Q., Qian, G., & Zhao, W. (2023). Solid- bioelectrochemical cancer detection: An overview of recent state nanopore array: Manufacturing and applications. Small, advances in emerging materials and technologies. Discover 19(9), 2205680. https://doi.org/10.1002/smll.202205680 Applied Sciences, 6, 47. https://doi.org/10.1007/s42452-024- [6] Restrepo-Pérez, L., Schmid, S., Dergaz, J., Alon, Y., Haaf, 05681-z
M., Dekker, C., & Aksimentiev, A. (2023). Nanopore detection [11] Huang, G., Willems, K., Soskine, M., Wloka, C., Maglia,
using supercharged polypeptide molecular carriers. Journal of G., & others. (2017). Electro-osmotic capture and ionic the American Chemical Society, 145(11), 6371–6382. https:// discrimination of peptide and protein biomarkers with FraC doi.org/10.1021/jacs.2c13465 nanopores. Nature Communications, 8, 935. https://doi. [7] Ding, T., Yang, J., Pan, V., Zhao, N., Lu, Z., Ke, Y., & Zhang, org/10.1038/s41467-017-01006-4
C. (2020). DNA nanotechnology assisted nanopore-based [12] Wan, Y., Hendra, C., Pratanwanich, P. N., & Göke, J. (2021). analysis. Nucleic Acids Research, 48(6), 2791–2806. https://doi. Beyond sequencing: Machine learning algorithms extract org/10.1093/nar/gkaa095 biology hidden in nanopore signal data. Trends in Genetics, [8] Wang, W., Xu, Y., Gao, R., Lu, R., Han, K., Wu, G., & Tan, W. 37(9), 876–889. https://doi.org/10.1016/j.tig.2021.09.001 (2020). Detection of SARS-CoV-2 in different types of clinical [13] Branton, D., Deamer, D., Marziali, A., Bayley, H., Benner, specimens. JAMA, 323(18), 1843–1844. https://doi.org/10.1001/ S. A., Butler, T., Di Ventra, M., Garaj, S., Hibbs, A., Huang, jama.2020.3786 X., Jovanovich, S. B., Krstic, P. S., Lindsay, S., Ling, X. [9] Chen, J., & Xu, F. (2023). Application of nanopore S., Mastrangelo, C. H., Meller, A., Oliver, J. S., Pershin, Y.
sequencing in the diagnosis and treatment of pulmonary V., Ramsey, J. M., ... Akeson, M. (2008). The potential and infections. Molecular Diagnosis & Therapy, 27(6), 685–701. challenges of nanopore sequencing. Nature Biotechnology, https://doi.org/10.1007/s40291-023-00669-8 26(10), 1146–1153. https://doi.org/10.1038/nbt.1495
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