Treatment with Increasing Amounts and for Various Durations of theAnticancer Agent Curcumol’s Effect on Stimulation of the Clearanceof HEP3b Xenograft Tumors and Decreasing of Jak3/Stat3 Signalingand Phosphorylation.
DOI:
https://doi.org/10.61173/4zw8bc46Keywords:
Curcumol, Indoleamine 2, 3-dioxygenase (IDO), Human Hepatocellular Carcinoma (HCC)Abstract
Human Hepatocellular Carcinoma is the second leading cause of cancer deaths, and it is undoubtedly easy to be infected
with a destructive lifestyle. However, humans’ immune systems will be effective for cancer treatment if there is no
suppression of T cells brought by Jak3/Stat3 molecular activities. Fortunately, Curcumol has been proven effective for
several cancer treatments and might also be helpful in HCC cancer treatment. This paper tends to prove that treatment
with increasing amounts and for various durations of the anticancer agent Curcumol will stimulate the clearance of
HEP3b xenograft tumors and decrease Jak3/Stat3 signaling and phosphorylation in the in vivo conditions. STAT3
phosphorylation and STAT3 mediated transcription will be measured by western blot analysis and STAT3 reporter assay.
HEP3b killing will be measured by MTT assay. In the experiment, the Positive control is Taxol, and the negative control
is PBS/DMSO. Each experiment will be repeated five times with different concentrations and various treatment duration
of Curcumol extractives, while all the experiments will be done in vivo. .
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