Long Noncoding-RNA Component of Mitochondrial RNA ProcessingEndoribonuclease Promotes Gastric Cancer Cell Multiplication,Migration, and Invasion
DOI:
https://doi.org/10.61173/mj96em41Keywords:
Noncoding-RNA, Mitochondrial RNA Processing Endoribonuclease, Gastric Cancer, Cell Multiplication, Cell Migration, Cell InvasionAbstract
Since RMRP was found to target microRNA-766-5p (miR-766-5p) in TNBC cells, it enhanced cell viability and
decreased apoptosis by silencing miR-766-5p. Additionally, RMRP has been proven to be highly involved in
either pathological or physiological processes. Therefore, the study aims to determine whether RMRP promotes
the multiplication, migration, and invasion of gastric cancer cells. The study will overexpress RMRP in GC1401
gastric cancer cells and measure cell growth by MTT assay, migration by wound healing assay, and Boyden chamber
assay. Positive control is another treatment previously shown to increase cancer cell growth and migration, such as
IQGAP3, which, beginning with the early stages of tumor growth, is markedly up-regulated in human stomach cancer.
Furthermore, the negative control is DMSO. The study measures miR206 by qRTPCR. If the solution gets dark through
MTT assay, RMRP promotes gastric cancer cell multiplication. If the migration rate is getting quicker according to the
wound healing assay, and the number of migration cells increases by Boyden chamber assay, RMRP promotes gastric
cancer cell migration. If miR-206 may affect the expression of HIF-1 to control cell growth and ECM buildup, RMRP
promotes gastric cancer cell invasion by acting as a miR-206 sponge for gastric cancer. The RNA component of miRNA
processing endoribonuclease, the novel biomarker for gastric cancer, promotes gastric cancer cell multiplication,
migration, and invasion by acting as a miR-206 loss of function in cell lines for gastric cancer.
References
[1] Hamashima, Chisato. “Current issues and future perspectives of gastric cancer screening.” World Journal of Gastroenterology: WJG 20.38 (2014): 13767.
[2] Wu, Hua-Hsi, Wen-chang Lin, and Kuo-Wang Tsai. “Advances in molecular biomarkers for gastric cancer: miRNAs as emerging novel cancer markers.” Expert reviews in molecular medicine 16 (2014).
[3] Durães, Cecília, et al. «Biomarkers for gastric cancer: prognostic, predictive or targets of therapy?.» Virchows Archiv 464.3 (2014): 367-378. Dean&Francis
[4] Shao, Yongfu, et al. “LncRNA-RMRP promotes carcinogenesis by acting as a miR-206 sponge and is used as a novel biomarker for gastric cancer.” Oncotarget 7.25 (2016): 37812.
[5] Hao, Qian, and Xiang Zhou. “The emerging role of long noncoding RNA RMRP in cancer development and targeted therapy.” Cancer Biology & Medicine 19.2 (2022): 140.
[6] Qi, Liqiang, et al. “Long Noncoding-RNA Component of Mitochondrial RNA Processing Endoribonuclease Promotes Carcinogenesis in Triple-Negative Breast Cancer Cells via the Competing Endogenous RNA Mechanism.” Journal of Breast Cancer 24.5 (2021): 428.
[7] Li, Jing, Zhenyu Xuan, and Changning Liu. “Long noncoding RNAs and complex human diseases.” International journal of molecular sciences 14.9 (2013): 18790-18808.
[8] Fatica, Alessandro, and Irene Bozzoni. “Long non-coding RNAs: new players in cell differentiation and development.” Nature Reviews Genetics 15.1 (2014): 7-21.
[9] Rosenbluh, Joseph, et al. “RMRP is a non-coding RNA essential for early murine development.” PloS one 6.10 (2011): e26270.
[10] Ghafouri-Fard, Soudeh, et al. “An update on the role of miR-379 in human disorders.” Biomedicine & Pharmacotherapy 139 (2021): 111553.
[11] Liu, W. H., et al. “LINC00641 regulates prostate cancer cell growth and apoptosis via the miR-365a-3p/VGLL4 axis.” Eur Rev Med Pharmacol Sci 25.1 (2021): 108-15.
[12] Bai, Hua, and Suhui Wu. “miR-451: a novel biomarker and potential therapeutic target for cancer.” OncoTargets and therapy 12 (2019): 11069.
[13] Zhou, Zhong-xin, et al. “miR-632 promotes laryngeal carcinoma cell proliferation, migration, and invasion through negative regulation of GSK3β.” Oncology research 28.1 (2020): 21.
[14] Wang, Lili, Hongguang Song, and Shiming Yang. “MicroRNA‐206 has a bright application prospect in the diagnosis of cases with oral cancer.” Journal of Cellular and Molecular Medicine 25.17 (2021): 8169-8173.
[15] Ghafouri-Fard, Soudeh, et al. “The impact of lncRNAs and miRNAs in regulation of function of cancer stem cells and progression of cancer.” Frontiers in Cell and Developmental Biology (2021): 2036.
[16] Wang, Xin, et al. “Regulation of laryngeal squamous cell cancer progression by the lncRNA RP11‐159K7. 2/miR‐206/ DNMT3A axis.” Journal of Cellular and Molecular Medicine 24.12 (2020): 6781-6795.
[17] Fang, Xin-yu, et al. “Long noncoding RNAs: novel insights into gastric cancer.” Cancer letters 356.2 (2015): 357-366.
[18] Song, Haojun, et al. “Long non-coding RNA expression profile in human gastric cancer and its clinical significances.” Journal of translational medicine 11.1 (2013): 1-10.
[19] Xia, Tian, et al. “Long noncoding RNA FER1L4 suppresses cancer cell growth by acting as a competing endogenous RNA and regulating PTEN expression.” Scientific reports 5.1 (2015): 1-9.
[20] Xie, Min, et al. “Long noncoding RNA HOXA-AS2 promotes gastric cancer proliferation by epigenetically silencing P21/PLK3/DDIT3 expression.” Oncotarget 6.32 (2015): 33587.
[21] Vistica VT, Skehan P, Scudiero D, Monks A, Pittman A, Boyd MR. 1991. Tetrazolium-based assays for cellular viability: a critical examination of selected parameters affecting formazan production. Cancer Res. 51(10):2515-20.
[22] Maehara Y, Anai H, Tamada R, Sugimachi K. 1987. The ATP assay is more sensitive than the succinate dehydrogenase inhibition test for predicting cell viability. European Journal of Cancer and Clinical Oncology. 23(3):273-276.
[23] Slater T, Sawyer B, Sträuli U. 1963. Studies on succinatetetrazolium reductase systems. Biochimica et Biophysica Acta. 77383-393.
[24] Berridge M, Tan A. 1993. Characterization of the Cellular Reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT): Subcellular Localization, Substrate Dependence, and Involvement of Mitochondrial Electron Transport in MTT Reduction. Archives of Biochemistry and Biophysics. 303(2):474-482.
[25] Cao, Yanchao, et al. “miR-206 inhibits cell proliferation and extracellular matrix accumulation by targeting hypoxiainducible factor 1-alpha (HIF-1α) in mesangial cells treated with high glucose.” Medical Science Monitor: International Medical Journal of Experimental and Clinical Research 25 (2019): 10036.
[26] Zheng, Liduan, et al. “miRNA-558 promotes gastric cancer progression through attenuating Smad4-mediated repression of heparanase expression.” Cell death & disease 7.9 (2016): e2382-e2382.
Downloads
Published
Issue
Section
License
Copyright (c) 2023 by the authors.
This work is licensed under a Creative Commons Attribution 4.0 International License.
