Osthole in Bushen Formula May Block HBV Gene Expression throughNotch and NF-κB Pathways
DOI:
https://doi.org/10.61173/32g9p963Keywords:
Osthole, Bushen formula, HBV gene, Notch, NF-κBAbstract
Osthole is a natural coumarin in the Bushen formulation. Previous studies reported that it has the potential to
downregulate Notch and NFκB, which are important signaling pathways in cells. Therefore, we predicted that ostiole
might affect HBV gene expression by down-regulating these two signaling pathways. This study provides experimental
protocols to apply osthole to mouse hepatocytes and detect the strength of Notch and NFκB signaling pathways in
the cells and HBV gene expression levels. In this work, all possible results were analyzed. Notch signal intensity was
measured by assaying NICD, IκB measured NFκB signal intensity, and RT-PCR measured HBV gene expression level.
The study of Osthole is meaningful for the research on Chinese medicine. This study will allow a better explanation of
the mechanism of Bush’s formula and explore the more generalized effects of herbal medicines
References
[1] WHO (World Health Organization) Global Hepatitis Report 2017. [(accessed on 4 April 2019)]; Available online: https:// www.who.int/hepatitis/publications/global-hepatitis-report2017/ en/
[2] GBD 2016 Disease and Injury Incidence and Prevalence Collaborators Global, regional, and national age-sex specific mortality for 264 causes of death, 1980–2016: A systematic analysis for the Global Burden of Disease Study 2016. Lancet. 2017;390:1151–1210. doi: 10.1016/S0140-6736(17)32152-9.
[3] Kennedy P.T.F., Litwin S., Dolman G.E., Bertoletti A., Mason W.S. Immune Tolerant Chronic Hepatitis B: The Unrecognized Risks. Viruses. 2017;9:96. doi: 10.3390/v9050096.
[4] Li M, Sun XH, Zhou ZH, Yu Z, Jin SG, Zhu XJ, Gao YQ. Beneficial therapeutic effect of Chinese herbal Bushen formula on CHB patients with mildly elevated alanine aminotransferase by down-regulating CD4+CD25+T cells. J Ethnopharmacol. 2013 Mar 27;146(2):614-22. doi: 10.1016/j.jep.2013.01.038. Epub 2013 Feb 7. PMID: 23395622.
[5] Zhang L, Yan R, Su R, Yang C, Liu S, Yu X, Chang X, Zhang S, Liu C, Xu M, Zeng W, Chen Y, Wang Q. Bioavailability enhancement of osthole after oral administration of Bushen Yizhi prescription extract to rats followed by Cnidium monnieri (L.) Cusson fruits extract in comparison to pure osthole at different doses. J Ethnopharmacol. 2014 Mar 14;152(2):266- 71. doi: 10.1016/j.jep.2013.09.045. Epub 2013 Oct 16. PMID: 24140583.
[6] Li, Y. Z., Sun, Z., Xu, H. R., Zhang, Q. G., & Zeng, C. Q. (2020). Osthole inhibits proliferation of kainic acidactivated BV2 cells by modulating the Notch signaling pathway. Molecular medicine reports, 22(5), 3759–3766. https://doi. org/10.3892/mmr.2020.11455
[7] Yu C, Li P, Qi D, Wang L, Qu HL, Zhang YJ, Wang XK, Fan HY. Osthole protects sepsis-induced acute kidney injury via down-regulating NF-κB signal pathway. Oncotarget. 2017 Jan 17;8(3):4796-4813. doi: 10.18632/oncotarget.13592. PMID: 27902475; PMCID: PMC5354872.
[8] Fan H, Gao Z, Ji K, Li X, Wu J, Liu Y, Wang X, Liang H, Liu Y, Li X, Liu P, Chen D, Zhao F. The in vitro and in vivo antiinflammatory effect of osthole, the major natural coumarin from Cnidium monnieri (L.) Cuss, via the blocking of the activation of the NF-κB and MAPK/p38 pathways. Phytomedicine. 2019 May;58:152864. doi: 10.1016/j.phymed.2019.152864. Epub 2019 Feb 18. PMID: 30878874. Dean&Francis
[9] Geisler F, Strazzabosco M. Emerging roles of Notch signaling in liver disease. Hepatology. 2015;61:382–392. doi: 10.1002/hep.27268.
[10] Lin, J., Gu, C., Shen, Z., Liu, Y., Wang, W., Tao, S., Cui, X., Liu, J., & Xie, Y. (2017). Hepatocyte nuclear factor 1α downregulates HBV gene expression and replication by activating the NF-κB signaling pathway. PloS one, 12(3), e0174017. https://doi.org/10.1371/journal.pone.0174017
[11] Feng J, Yang G, Liu Y, Gao Y, Zhao M, Bu Y, Yuan H, Yuan Y, Yun H, Sun M, Gao H, Zhang S, Liu Z, Yin M, Song X, Miao Z, Lin Z, Zhang X. LncRNA PCNAP1 modulates hepatitis B virus replication and enhances tumor growth of liver cancer. Theranostics 2019; 9(18):5227-5245. doi:10.7150/thno.34273. Available from https://www.thno.org/v09p5227.htm
[12] Zhang W, Chen J, Wu M, Zhang X, Zhang M, Yue L. et al. PRMT5 restricts hepatitis B virus replication through epigenetic repression of covalently closed circular DNA transcription and interference with pregenomic RNA encapsidation. Hepatology. 2017;66:398-415
[13] Li M, Sun XH, Zhou ZH, Yu Z, Jin SG, Zhu XJ, Gao YQ. Beneficial therapeutic effect of Chinese herbal Bushen formula on CHB patients with mildly elevated alanine aminotransferase by down-regulating CD4+CD25+T cells. J Ethnopharmacol. 2013 Mar 27;146(2):614-22. doi: 10.1016/j.jep.2013.01.038. Epub 2013 Feb 7. PMID: 23395622. Dean&Francis
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