Quercetin Inhibits Colorectal Cancer by Enhancing Apoptosis viaPI3K/Akt Pathway
DOI:
https://doi.org/10.61173/mdqcjr04Keywords:
quercetin, colorectal cancer, apoptosis, phospho-AKTAbstract
CRC is the second leading cancer globally, with a high lethality rate. The primary treatment of CRC is still surgery and
chemotherapy with limited therapeutic effects. Quercetin, a component of traditional Chinese medicine, has shown its
ability to anti-tumor and induce cell apoptosis through PI3K/AKT signalling pathway. This study aims to figure out how
concentrations of quercetin and different treatment durations will influence the effects of induction of apoptosis. The
experiments will use CL40 cells. Cell apoptosis is measured by flow cytometry, and cell growth by MTT assay. The
phospho-AKT level is measured by anti-p-AKT Western Blot. Our research will determine the relationship between
therapeutic effects, drug concentrations, and treatment durations. Further studies on the side effects of quercetin and
methods to mitigate the side effects in the systematic use of quercetin should be done. Moreover, a more specific
mechanism for the function of quercetin is waiting to clarify
References
[1] Dekker, Evelien et al. “Colorectal cancer.” Lancet (London, England) vol. 394,10207 (2019): 1467-1480.
[2] Deng, Xiao-Hui et al. “Effects of quercetin on the proliferation of breast cancer cells and expression of surviving in vitro.” Experimental and therapeutic medicine vol. 6,5 (2013): 1155-1158.
[3] Lopez, Andrea, et al. “Co-targeting of BAX and BCL-XL proteins broadly overcomes resistance to apoptosis in cancer.” Nature communications 13.1 (2022): 1-18.
[4] Jahanafrooz, Zohreh, et al. “Colon cancer therapy by focusing on colon cancer stem cells and their tumor microenvironment.” Journal of cellular physiology 235.5 (2020): 4153-4166.
[5] Na, Song, et al. “Study on the molecular mechanism of nightshade in the treatment of colon cancer.” Bioengineered 13.1 (2022): 1575-1589.
[6] Tang, Si-Min, et al. “Pharmacological basis and new insights of quercetin action in respect to its anti-cancer effects.” Biomedicine & Pharmacotherapy 121 (2020): 109604.
[7] Wen, Jun, et al. “ACLY facilitates colon cancer cell metastasis by CTNNB1.” Journal of Experimental & Clinical Cancer Research 38.1 (2019): 1-12.
[8] Pommerenke C, Rand U, Uphoff CC, Nagel S, Zaborski M, Hauer V, Kaufmann M, Meyer C, Denkmann SA, Riese P, Eschke K, Kim Y, Safranko ZM, Kurolt IC, Markotic A, Cicin- Sain L, Steenpass L. Identification of cell lines CL-14, CL- 40 and CAL-51 as suitable models for SARS-CoV-2 infection studies. PLoS One. 2021 Aug 2;16(8).
[9] Blancher C, Jones A. SDS -PAGE and Western Blotting Techniques. Methods Mol Med. 2001; 57:145-62.
[10] Kumar P, Nagarajan A, Uchil PD. Analysis of Cell Viability by the MTT Assay. Cold Spring Harb Protoc. 2018 Jun 1;2018(6).
Downloads
Published
Issue
Section
License
Copyright (c) 2023 by the authors.
This work is licensed under a Creative Commons Attribution 4.0 International License.
