The Effect of Agrimol B on A549 Lung Cancer Cells by Blocking Mitochondria Biogenesis

Abstract

Purpose: Since Agrimol B can combine with PGC-1α, impair mitochondrial biogenesis, and promote tumor apoptosis, thus inhibiting colon carcinoma progression, I predict that increasing concentrations and treatment durations with Agrimol B kills A549 lung cancer cells by blocking mitochondria biogenesis.

Methods: The study will measure cell killing by MTT assay, cell migration inhibition by Boyden chamber assay, and killing of cancer cells in vivo by measuring tumor size in A549 xenograft mice and confocal microscopy with mitotracker stain to measure reduction in mitochondria. The positive control is Anlotinib Hydrochloride. The negative control is DMSO/PBS.

Possible results: There are three main possible results: (1) Agrimol B kills A549 cancer cells. It reduces mitochondria of A549 lung cancer cells, inhibits cell migration, and kills A549 lung cancer cells (2) Agrimol B promotes A549 cancer cells. It promotes mitochondria of A549 lung cancer cells and cell migration. (3) Agrimol B does not significantly affect the mitochondria biogenesis and migration of A549 cancer cells.

Conclusion: The result of the study will provide important insight into the effect of Agrimol B on A549 cancer cells. It would increase our understanding of the treatment of cancer with Chinese medicine at the molecular level and improve human health.