The Potential of Gene Editing Technology in Agriculture: Cre-Loxp and CRISPR-Cas9

Abstract

In recent years, gene editing technology has made breakthrough progress. This article uses Cre-loxP and CRISPR-Cas9 as examples to explore its application prospects in the agricultural field. Current research has made substantial progress in using these technologies for genetic modification of crops. However, there is currently a lack of comparative studies on Cre-loxP and CRISPR-Cas9. This article analyzes the Cre-loxP and CRISPR-Cas9 systems that rely on Cre enzyme and Cas9 endonuclease for targeted cleavage respectively, summarizes the mechanism and functional research of the two technologies, and discusses their application in agricultural gene editing. The shortcomings and challenges of both technologies are discussed, and some suggestions for improvements are made. The significance of this study is to provide a comprehensive review of the development trends of gene editing in the agricultural field. The findings contribute to the existing literature by highlighting the potential of Cre-loxP and CRISPR-Cas9 in the genetic modification of crops. However, issues such as technology limitations and off-targeting still require close attention. All these challenges and shortcomings will be addressed in future studies to promote the application of CRISPR-Cas9 and Cre-loxP in agriculture.