Using fluorescence, PET, and fMRI to detect the alteration of patterns and activation of specific neurons in the subiculum in response to hippocampus neurogenesis

Abstract

In this paper, the questions started with “What will happen to the brain if AHN(adult hippocampus neurogenesis) increases” and then going further on the question became “How would certain neurons in the hypothalamus and subiculum change if AHN increases”. Therefore, this paper will address this question and propose three experiments and a conclusion. For the experiments, PET and fMRI are used to scan the brain and see the levels of glucose and therefore the activation of neurons. Furthermore, FosTRAP2 with fluoresce allows a better in-depth look at the parts where neurons were active, or in this case, activated due to stress. The three experiments should show more activity/fluorescence in the hypothalamus and subiculum in groups with neurogenesis compared to the control groups with CORT injection. In conclusion, if the results are in accordance with the hypothesis, this allows a better insight into the mechanism and link between anxiety and AHN. This will also mean a correlation with the subiculum, HPA, and AHN.