Functional Analysis of the Promoter pBnMYB69-1 in Brassica Napus
DOI:
https://doi.org/10.61173/nj2nbv28Keywords:
Brassica napus, promoter, function, pBnMYB69Abstract
MYB transcription factors play a crucial role in regulating plant development and adaptation. Brassica napus, a major oil crop, often faces challenges such as lodging and diseases. In this study, using the genome of Brassica napus as a template, we cloned a 2000 bp promoter sequence upstream of the start codon of the pBnMYB69-1 gene. Prediction and analysis of cis-regulatory elements revealed that the promoter contains essential core regulatory elements of eukaryotic promoters, such as the TATA box and CAAT box. Additionally, it includes various functional elements, such as light-responsive elements (ATCT-motif, Box 4, TCT-motif), anaerobic induction elements (ARE), methyl jasmonate (MeJA)-responsive elements (CGTCA-motif, TGACG-motif), and gibberellin-responsive elements (P-box). These findings provide a solid foundation for the application of this promoter in transgenic improvement of crop quality and the artificial creation of germplasm resources in Brassica napus.References
[1] Ooms G, Bains A, Burrell M, et al. Genetic manipulation in cultivars of oilseed rape( Brassica napus) using Agrobacterium[J]. Theoretical and Applied Genetics, 1985, 71( 2) : 325 - 329.
[2] Yao Q, Lin Y. Cloning and functional analysis of a promoter with temporal - spatial expressing differentiation in rice root[J]. Journal of Agricultural Biotechnology, 2011, 19( 2) : 214 - 220.
[3] Battraw M J, Hall T C. Histochemical analysis of CaMV 35S promoter - β - glucuronidase gene expression in transgenic rice plants [J]. Plant Molecular Biology, 1990, 15( 4) : 527 - 538.
[4] Ebert P R, Ha S B, An G. Identification of an essential upstream element in the nopaline synthase promoter by stable and transient assays[J]. Proceedings of the National Academy of Sciences, 1987, 84( 16) : 5745 - 5749.
[5] Odell J T, Nagy F, Chua N H. Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter[J]. Nature, 1985, 313( 6005) : 810.
[6] Zhu Yuxian, Li Yi. Modern Molecular Biology[M]. Beijing: Higher Education Press, 2005.
[7] Irie T, Honda Y, Hirano T, et al. Stable transformation of Pleurotus ostreatus to hygromycin B resistance using Lentinus edodes GPD expression signals[J]. Applied Microbiology and Biotechnology, 2001, 56( 5 /6) : 707 - 709.
[8] Feller, A., Machemer, K., Braun, E. L., and Grotewold, E. (2011). Evolutionary and comparative analysis ofMYB and bHLH plant transcription factors. Plant J. 66 (1), 94–116. doi: 10.1111/j.1365-313X.2010.04459.x
[9] Hajiebrahimi, A., Owji, H., and Hemmati, S. (2017). Genome-wide identification, functional prediction, and evolutionary analysis of the R2R3-MYB superfamily in Brassica napus. Genome 60 (10), 797–814. doi: 10.1139/gen-2017-0059
[10] Baldoni, E., Genga, A., and Cominelli, E. (2015). Plant MYB transcription factors: Their role in drought response mechanisms. Int. J. Mol. Sci. 16 (7), 15811–15851. doi: 10.3390/ ijms160715811
[11] Wei, X.,Shan, T.,Hong, Y.,Xu, H.,Liu,X., andZhang,Z. (2017). TaPIMP2, apathogen-induced MYB protein in wheat, contributes to host resistance to common root rot caused by Bipolaris sorokinian. Sci. Rep. 7, 1754. doi: 10.1038/s41598- 017-01918-7
[12] Dubos, C., Stracke, R., Grotewold, E., Weisshaar, B., Martin, C., and Lepiniec, L. (2010). MYB transcription factors in Arabidopsis. Trends Plant Sci. 15 (10), 573–581. doi: 10.1016/ j.tplants.2010.06.005
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